Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine

The natural hosts of Orthohantaviruses are rodents, soricomorphs and bats, it is well known that they may cause serious or even fatal diseases among humans worldwide. virus persistent animals shed via urine, saliva feces throughout the entirety their lives. We aim to identify effectiveness hantavirus detection in rodent tissue samples urine originating from naturally infected rodents. Initially, were trapped at five distinct locations Transdanubian region Hungary. Lung, liver, kidney obtained 163 deceased animals. All organs tested using nested reverse transcription polymerase chain reaction (nRT-PCR). Furthermore, sera examined for IgG antibodies against Dobrava–Belgrade (DOBV) Puumala (PUUV) by Western blot assay. hantaviruses and/or nucleic acid detected 25 (15.3%) cases. Among Apodemus, Myodes, Microtus species, DOBV, PUUV Tula (TULV) clearly identified. Amid PCR-positive samples, viruses was most effectively (100%), while only 55% screened lung tissues positive. Interestingly, three out 20 positive when nRT-PCR. Moreover, rodents seropositive without detectable any organs.